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    Greiner Bio plates
    Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sapphire+microplate/Sapphire+Microplate+384+Well+Pp+Pcr/pm41782368-328-6-7
    Average 94 stars, based on 12 article reviews
    plates - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    CRISPR:

    Article Title: CLASP2 safeguards hematopoietic stem cell properties during mouse and fish development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES ThermoFisher Cat#: 15630080 Protease Inhibitor Cocktail Thermo Scientific Cat#: A32955 Bafilomycin A1 (BafA, 100nM) Invitrogen Cat#: tlrl-baf1 Tunicamycin Sigma-Aldrich Cat#: T7765 Fetal calf serum Sigma Cat#: F7524 L-Glutamine 200 mM (100ml) Life Technologies Cat#: 25030-024 L-Glutamine Invitrogen Cat#: 25030-032 Sodium pyruvate ThermoFisher Cat#: 11360070 Sodium pyruvate Invitrogen Cat#: 11360-039 2 -mercaptoethanol (0.1mM) Gibco Cat#: 11528926 Non-fat powder milk Sigma Cat#: 70166-500G PVDF membranes Millipore Cat#: IPVH00010 LIF Sigma-Aldrich Cat#: PD0325901 CHIR Tocris Biochemicals Cat#: 4423-10 PD Stemgent Cat#: 04-0006 Endo H NEB Cat#: P0702S Pngase F NEB Cat#: P0704S IRDyes 800CW and 600CW Li-cor N/A Enhanced chemiluminescence detection (ECL) GE Healthcare Cat#: RPN2236 Fluorescent detection (Odyssey Imaging System Licor Cat#: 926-68071 (goat anti-rabbit, IR dye 680 CW) Cat#: 926-32210 (goat anti-mouse, IR dye 800 CW) Ambion MEGAscript T7 transcription kit Life Technologies Cat#: AM1333 pCS2-nCas9 n plasmid Addgene Cat#: 47929 Clasp2 morpholino GeneTools, LCC N/A pCS2+ plasmid Kind gift from Prof. den Hertog (Hubrecht Institute) N/A BD Cytofix/CytopermTM BD Bioscience Cat#: 554714 RNeasy Mini kit Qiagen Cat#: 74104 SuperScript III Reverse Transcriptase Life Technologies Cat#: 18080044 SuperScript IV Reverse Transcriptase Thermo Fisher Cat#: 18-090-050 GFP-GM130 mRNA (Pouthas et al., 2008) mRNA kindly provided by Prof. Bakkers (Hubrecht Institute) N/A Experimental models: Organisms/strains Mouse: C57BL/6J (CD45.2) The Jackson Laboratory JAX: 000664 Mouse: Clasp2+/- (C57BL/6) Kind gift from Prof. Galjart (ErasmusMC, Rotterdam, NL) (Drabek et al., 2012) Zebrafish: mpeg1.4:mCherry/mpx:eGFP This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-4bp] This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-10bp] This paper N/A Zebrafish: clasp2mut[-4bp] This paper N/A Zebrafish: clasp2mut[-10bp] This paper N/A Zebrafish: AB EZRC Item #: 1175.1 Zebrafish: TL EZRC Item #: 1174.1 (Continued on next page) e3 Cell Reports 39, 110957, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MS-5 stromal line Kind gift from Drs. Dusanter and Lauret (Cochin Institute) N/A Oligonucleotides Morpholinos GeneTools, LCC N/A Primers for genotyping, CRISPR/Cas9 genome editing, semi-quantitative PCR, RT-qPCR, RT-PCR, ISH, see Table S1 IDT N/A Software and algorithms Excel Microsoft N/A Fiji (ImageJ) Fiji https://fiji.sc FlowJo FlowJo https://www.flowjo.com/ Prism7 GraphPad Software GraphPad Software Imaris X Oxford Instruments N/A CellQuest software BD Bioscience N/A CytExpert software Beckman Coulter N/A Chopchop N/A https://chopchop.cbu.uib.no/ SnapGene GSL Biotech LLC N/A Ensembl EMBL-EBI https://www.ensembl.org/ index.html Other LSM700 Confocal Microscope Zeiss N/A Leica fluorescent stereomicroscope MZ 16 FA Leica N/A Leica SP8 confocal microscope Leica N/A Aria III flow cytometer BD Bioscience N/A FACSCalibur BD Bioscience N/A Jazz BD FACSJazz BD Bioscience N/A FACSDiva BD Bioscience N/A BD Influx Cell Sorter BD Bioscience N/A CytoFLEX benchtop flow cytometer Beckman Coulter N/A Hamamatsu Flash4 LT digital camera Hamamatsu N/A BIO-RAD C1000 Touch Thermal Cycler, CFX384TM Real-Time System BIO-RAD N/A ThermalSealRTTM Greiner Cat#: 676044 SAPPHIRE MICROPLATE, 384 WELL Greiner Cat#: 785201 Mmessage Mmachine Sp6 Kit Thermo Fisher Scientific Cat#: 10086184 Four-well imaging dish (Muwells) (Weijts et al., 2017) N/A Cell strainers 40 um Blue VWR Cat#: 734-0002 Adhesion slides, SuperFrost Plus VWR Cat#: 631-0108 Durapore Membrane Filters (0.65 mm) Millipore Cat#: DVPP02500 ..

    Polymerase Chain Reaction:

    Article Title: CLASP2 safeguards hematopoietic stem cell properties during mouse and fish development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES ThermoFisher Cat#: 15630080 Protease Inhibitor Cocktail Thermo Scientific Cat#: A32955 Bafilomycin A1 (BafA, 100nM) Invitrogen Cat#: tlrl-baf1 Tunicamycin Sigma-Aldrich Cat#: T7765 Fetal calf serum Sigma Cat#: F7524 L-Glutamine 200 mM (100ml) Life Technologies Cat#: 25030-024 L-Glutamine Invitrogen Cat#: 25030-032 Sodium pyruvate ThermoFisher Cat#: 11360070 Sodium pyruvate Invitrogen Cat#: 11360-039 2 -mercaptoethanol (0.1mM) Gibco Cat#: 11528926 Non-fat powder milk Sigma Cat#: 70166-500G PVDF membranes Millipore Cat#: IPVH00010 LIF Sigma-Aldrich Cat#: PD0325901 CHIR Tocris Biochemicals Cat#: 4423-10 PD Stemgent Cat#: 04-0006 Endo H NEB Cat#: P0702S Pngase F NEB Cat#: P0704S IRDyes 800CW and 600CW Li-cor N/A Enhanced chemiluminescence detection (ECL) GE Healthcare Cat#: RPN2236 Fluorescent detection (Odyssey Imaging System Licor Cat#: 926-68071 (goat anti-rabbit, IR dye 680 CW) Cat#: 926-32210 (goat anti-mouse, IR dye 800 CW) Ambion MEGAscript T7 transcription kit Life Technologies Cat#: AM1333 pCS2-nCas9 n plasmid Addgene Cat#: 47929 Clasp2 morpholino GeneTools, LCC N/A pCS2+ plasmid Kind gift from Prof. den Hertog (Hubrecht Institute) N/A BD Cytofix/CytopermTM BD Bioscience Cat#: 554714 RNeasy Mini kit Qiagen Cat#: 74104 SuperScript III Reverse Transcriptase Life Technologies Cat#: 18080044 SuperScript IV Reverse Transcriptase Thermo Fisher Cat#: 18-090-050 GFP-GM130 mRNA (Pouthas et al., 2008) mRNA kindly provided by Prof. Bakkers (Hubrecht Institute) N/A Experimental models: Organisms/strains Mouse: C57BL/6J (CD45.2) The Jackson Laboratory JAX: 000664 Mouse: Clasp2+/- (C57BL/6) Kind gift from Prof. Galjart (ErasmusMC, Rotterdam, NL) (Drabek et al., 2012) Zebrafish: mpeg1.4:mCherry/mpx:eGFP This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-4bp] This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-10bp] This paper N/A Zebrafish: clasp2mut[-4bp] This paper N/A Zebrafish: clasp2mut[-10bp] This paper N/A Zebrafish: AB EZRC Item #: 1175.1 Zebrafish: TL EZRC Item #: 1174.1 (Continued on next page) e3 Cell Reports 39, 110957, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MS-5 stromal line Kind gift from Drs. Dusanter and Lauret (Cochin Institute) N/A Oligonucleotides Morpholinos GeneTools, LCC N/A Primers for genotyping, CRISPR/Cas9 genome editing, semi-quantitative PCR, RT-qPCR, RT-PCR, ISH, see Table S1 IDT N/A Software and algorithms Excel Microsoft N/A Fiji (ImageJ) Fiji https://fiji.sc FlowJo FlowJo https://www.flowjo.com/ Prism7 GraphPad Software GraphPad Software Imaris X Oxford Instruments N/A CellQuest software BD Bioscience N/A CytExpert software Beckman Coulter N/A Chopchop N/A https://chopchop.cbu.uib.no/ SnapGene GSL Biotech LLC N/A Ensembl EMBL-EBI https://www.ensembl.org/ index.html Other LSM700 Confocal Microscope Zeiss N/A Leica fluorescent stereomicroscope MZ 16 FA Leica N/A Leica SP8 confocal microscope Leica N/A Aria III flow cytometer BD Bioscience N/A FACSCalibur BD Bioscience N/A Jazz BD FACSJazz BD Bioscience N/A FACSDiva BD Bioscience N/A BD Influx Cell Sorter BD Bioscience N/A CytoFLEX benchtop flow cytometer Beckman Coulter N/A Hamamatsu Flash4 LT digital camera Hamamatsu N/A BIO-RAD C1000 Touch Thermal Cycler, CFX384TM Real-Time System BIO-RAD N/A ThermalSealRTTM Greiner Cat#: 676044 SAPPHIRE MICROPLATE, 384 WELL Greiner Cat#: 785201 Mmessage Mmachine Sp6 Kit Thermo Fisher Scientific Cat#: 10086184 Four-well imaging dish (Muwells) (Weijts et al., 2017) N/A Cell strainers 40 um Blue VWR Cat#: 734-0002 Adhesion slides, SuperFrost Plus VWR Cat#: 631-0108 Durapore Membrane Filters (0.65 mm) Millipore Cat#: DVPP02500 ..

    In Situ Hybridization:

    Article Title: CLASP2 safeguards hematopoietic stem cell properties during mouse and fish development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES ThermoFisher Cat#: 15630080 Protease Inhibitor Cocktail Thermo Scientific Cat#: A32955 Bafilomycin A1 (BafA, 100nM) Invitrogen Cat#: tlrl-baf1 Tunicamycin Sigma-Aldrich Cat#: T7765 Fetal calf serum Sigma Cat#: F7524 L-Glutamine 200 mM (100ml) Life Technologies Cat#: 25030-024 L-Glutamine Invitrogen Cat#: 25030-032 Sodium pyruvate ThermoFisher Cat#: 11360070 Sodium pyruvate Invitrogen Cat#: 11360-039 2 -mercaptoethanol (0.1mM) Gibco Cat#: 11528926 Non-fat powder milk Sigma Cat#: 70166-500G PVDF membranes Millipore Cat#: IPVH00010 LIF Sigma-Aldrich Cat#: PD0325901 CHIR Tocris Biochemicals Cat#: 4423-10 PD Stemgent Cat#: 04-0006 Endo H NEB Cat#: P0702S Pngase F NEB Cat#: P0704S IRDyes 800CW and 600CW Li-cor N/A Enhanced chemiluminescence detection (ECL) GE Healthcare Cat#: RPN2236 Fluorescent detection (Odyssey Imaging System Licor Cat#: 926-68071 (goat anti-rabbit, IR dye 680 CW) Cat#: 926-32210 (goat anti-mouse, IR dye 800 CW) Ambion MEGAscript T7 transcription kit Life Technologies Cat#: AM1333 pCS2-nCas9 n plasmid Addgene Cat#: 47929 Clasp2 morpholino GeneTools, LCC N/A pCS2+ plasmid Kind gift from Prof. den Hertog (Hubrecht Institute) N/A BD Cytofix/CytopermTM BD Bioscience Cat#: 554714 RNeasy Mini kit Qiagen Cat#: 74104 SuperScript III Reverse Transcriptase Life Technologies Cat#: 18080044 SuperScript IV Reverse Transcriptase Thermo Fisher Cat#: 18-090-050 GFP-GM130 mRNA (Pouthas et al., 2008) mRNA kindly provided by Prof. Bakkers (Hubrecht Institute) N/A Experimental models: Organisms/strains Mouse: C57BL/6J (CD45.2) The Jackson Laboratory JAX: 000664 Mouse: Clasp2+/- (C57BL/6) Kind gift from Prof. Galjart (ErasmusMC, Rotterdam, NL) (Drabek et al., 2012) Zebrafish: mpeg1.4:mCherry/mpx:eGFP This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-4bp] This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-10bp] This paper N/A Zebrafish: clasp2mut[-4bp] This paper N/A Zebrafish: clasp2mut[-10bp] This paper N/A Zebrafish: AB EZRC Item #: 1175.1 Zebrafish: TL EZRC Item #: 1174.1 (Continued on next page) e3 Cell Reports 39, 110957, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MS-5 stromal line Kind gift from Drs. Dusanter and Lauret (Cochin Institute) N/A Oligonucleotides Morpholinos GeneTools, LCC N/A Primers for genotyping, CRISPR/Cas9 genome editing, semi-quantitative PCR, RT-qPCR, RT-PCR, ISH, see Table S1 IDT N/A Software and algorithms Excel Microsoft N/A Fiji (ImageJ) Fiji https://fiji.sc FlowJo FlowJo https://www.flowjo.com/ Prism7 GraphPad Software GraphPad Software Imaris X Oxford Instruments N/A CellQuest software BD Bioscience N/A CytExpert software Beckman Coulter N/A Chopchop N/A https://chopchop.cbu.uib.no/ SnapGene GSL Biotech LLC N/A Ensembl EMBL-EBI https://www.ensembl.org/ index.html Other LSM700 Confocal Microscope Zeiss N/A Leica fluorescent stereomicroscope MZ 16 FA Leica N/A Leica SP8 confocal microscope Leica N/A Aria III flow cytometer BD Bioscience N/A FACSCalibur BD Bioscience N/A Jazz BD FACSJazz BD Bioscience N/A FACSDiva BD Bioscience N/A BD Influx Cell Sorter BD Bioscience N/A CytoFLEX benchtop flow cytometer Beckman Coulter N/A Hamamatsu Flash4 LT digital camera Hamamatsu N/A BIO-RAD C1000 Touch Thermal Cycler, CFX384TM Real-Time System BIO-RAD N/A ThermalSealRTTM Greiner Cat#: 676044 SAPPHIRE MICROPLATE, 384 WELL Greiner Cat#: 785201 Mmessage Mmachine Sp6 Kit Thermo Fisher Scientific Cat#: 10086184 Four-well imaging dish (Muwells) (Weijts et al., 2017) N/A Cell strainers 40 um Blue VWR Cat#: 734-0002 Adhesion slides, SuperFrost Plus VWR Cat#: 631-0108 Durapore Membrane Filters (0.65 mm) Millipore Cat#: DVPP02500 ..

    Software:

    Article Title: CLASP2 safeguards hematopoietic stem cell properties during mouse and fish development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES ThermoFisher Cat#: 15630080 Protease Inhibitor Cocktail Thermo Scientific Cat#: A32955 Bafilomycin A1 (BafA, 100nM) Invitrogen Cat#: tlrl-baf1 Tunicamycin Sigma-Aldrich Cat#: T7765 Fetal calf serum Sigma Cat#: F7524 L-Glutamine 200 mM (100ml) Life Technologies Cat#: 25030-024 L-Glutamine Invitrogen Cat#: 25030-032 Sodium pyruvate ThermoFisher Cat#: 11360070 Sodium pyruvate Invitrogen Cat#: 11360-039 2 -mercaptoethanol (0.1mM) Gibco Cat#: 11528926 Non-fat powder milk Sigma Cat#: 70166-500G PVDF membranes Millipore Cat#: IPVH00010 LIF Sigma-Aldrich Cat#: PD0325901 CHIR Tocris Biochemicals Cat#: 4423-10 PD Stemgent Cat#: 04-0006 Endo H NEB Cat#: P0702S Pngase F NEB Cat#: P0704S IRDyes 800CW and 600CW Li-cor N/A Enhanced chemiluminescence detection (ECL) GE Healthcare Cat#: RPN2236 Fluorescent detection (Odyssey Imaging System Licor Cat#: 926-68071 (goat anti-rabbit, IR dye 680 CW) Cat#: 926-32210 (goat anti-mouse, IR dye 800 CW) Ambion MEGAscript T7 transcription kit Life Technologies Cat#: AM1333 pCS2-nCas9 n plasmid Addgene Cat#: 47929 Clasp2 morpholino GeneTools, LCC N/A pCS2+ plasmid Kind gift from Prof. den Hertog (Hubrecht Institute) N/A BD Cytofix/CytopermTM BD Bioscience Cat#: 554714 RNeasy Mini kit Qiagen Cat#: 74104 SuperScript III Reverse Transcriptase Life Technologies Cat#: 18080044 SuperScript IV Reverse Transcriptase Thermo Fisher Cat#: 18-090-050 GFP-GM130 mRNA (Pouthas et al., 2008) mRNA kindly provided by Prof. Bakkers (Hubrecht Institute) N/A Experimental models: Organisms/strains Mouse: C57BL/6J (CD45.2) The Jackson Laboratory JAX: 000664 Mouse: Clasp2+/- (C57BL/6) Kind gift from Prof. Galjart (ErasmusMC, Rotterdam, NL) (Drabek et al., 2012) Zebrafish: mpeg1.4:mCherry/mpx:eGFP This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-4bp] This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-10bp] This paper N/A Zebrafish: clasp2mut[-4bp] This paper N/A Zebrafish: clasp2mut[-10bp] This paper N/A Zebrafish: AB EZRC Item #: 1175.1 Zebrafish: TL EZRC Item #: 1174.1 (Continued on next page) e3 Cell Reports 39, 110957, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MS-5 stromal line Kind gift from Drs. Dusanter and Lauret (Cochin Institute) N/A Oligonucleotides Morpholinos GeneTools, LCC N/A Primers for genotyping, CRISPR/Cas9 genome editing, semi-quantitative PCR, RT-qPCR, RT-PCR, ISH, see Table S1 IDT N/A Software and algorithms Excel Microsoft N/A Fiji (ImageJ) Fiji https://fiji.sc FlowJo FlowJo https://www.flowjo.com/ Prism7 GraphPad Software GraphPad Software Imaris X Oxford Instruments N/A CellQuest software BD Bioscience N/A CytExpert software Beckman Coulter N/A Chopchop N/A https://chopchop.cbu.uib.no/ SnapGene GSL Biotech LLC N/A Ensembl EMBL-EBI https://www.ensembl.org/ index.html Other LSM700 Confocal Microscope Zeiss N/A Leica fluorescent stereomicroscope MZ 16 FA Leica N/A Leica SP8 confocal microscope Leica N/A Aria III flow cytometer BD Bioscience N/A FACSCalibur BD Bioscience N/A Jazz BD FACSJazz BD Bioscience N/A FACSDiva BD Bioscience N/A BD Influx Cell Sorter BD Bioscience N/A CytoFLEX benchtop flow cytometer Beckman Coulter N/A Hamamatsu Flash4 LT digital camera Hamamatsu N/A BIO-RAD C1000 Touch Thermal Cycler, CFX384TM Real-Time System BIO-RAD N/A ThermalSealRTTM Greiner Cat#: 676044 SAPPHIRE MICROPLATE, 384 WELL Greiner Cat#: 785201 Mmessage Mmachine Sp6 Kit Thermo Fisher Scientific Cat#: 10086184 Four-well imaging dish (Muwells) (Weijts et al., 2017) N/A Cell strainers 40 um Blue VWR Cat#: 734-0002 Adhesion slides, SuperFrost Plus VWR Cat#: 631-0108 Durapore Membrane Filters (0.65 mm) Millipore Cat#: DVPP02500 ..

    Microscopy:

    Article Title: CLASP2 safeguards hematopoietic stem cell properties during mouse and fish development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES ThermoFisher Cat#: 15630080 Protease Inhibitor Cocktail Thermo Scientific Cat#: A32955 Bafilomycin A1 (BafA, 100nM) Invitrogen Cat#: tlrl-baf1 Tunicamycin Sigma-Aldrich Cat#: T7765 Fetal calf serum Sigma Cat#: F7524 L-Glutamine 200 mM (100ml) Life Technologies Cat#: 25030-024 L-Glutamine Invitrogen Cat#: 25030-032 Sodium pyruvate ThermoFisher Cat#: 11360070 Sodium pyruvate Invitrogen Cat#: 11360-039 2 -mercaptoethanol (0.1mM) Gibco Cat#: 11528926 Non-fat powder milk Sigma Cat#: 70166-500G PVDF membranes Millipore Cat#: IPVH00010 LIF Sigma-Aldrich Cat#: PD0325901 CHIR Tocris Biochemicals Cat#: 4423-10 PD Stemgent Cat#: 04-0006 Endo H NEB Cat#: P0702S Pngase F NEB Cat#: P0704S IRDyes 800CW and 600CW Li-cor N/A Enhanced chemiluminescence detection (ECL) GE Healthcare Cat#: RPN2236 Fluorescent detection (Odyssey Imaging System Licor Cat#: 926-68071 (goat anti-rabbit, IR dye 680 CW) Cat#: 926-32210 (goat anti-mouse, IR dye 800 CW) Ambion MEGAscript T7 transcription kit Life Technologies Cat#: AM1333 pCS2-nCas9 n plasmid Addgene Cat#: 47929 Clasp2 morpholino GeneTools, LCC N/A pCS2+ plasmid Kind gift from Prof. den Hertog (Hubrecht Institute) N/A BD Cytofix/CytopermTM BD Bioscience Cat#: 554714 RNeasy Mini kit Qiagen Cat#: 74104 SuperScript III Reverse Transcriptase Life Technologies Cat#: 18080044 SuperScript IV Reverse Transcriptase Thermo Fisher Cat#: 18-090-050 GFP-GM130 mRNA (Pouthas et al., 2008) mRNA kindly provided by Prof. Bakkers (Hubrecht Institute) N/A Experimental models: Organisms/strains Mouse: C57BL/6J (CD45.2) The Jackson Laboratory JAX: 000664 Mouse: Clasp2+/- (C57BL/6) Kind gift from Prof. Galjart (ErasmusMC, Rotterdam, NL) (Drabek et al., 2012) Zebrafish: mpeg1.4:mCherry/mpx:eGFP This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-4bp] This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-10bp] This paper N/A Zebrafish: clasp2mut[-4bp] This paper N/A Zebrafish: clasp2mut[-10bp] This paper N/A Zebrafish: AB EZRC Item #: 1175.1 Zebrafish: TL EZRC Item #: 1174.1 (Continued on next page) e3 Cell Reports 39, 110957, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MS-5 stromal line Kind gift from Drs. Dusanter and Lauret (Cochin Institute) N/A Oligonucleotides Morpholinos GeneTools, LCC N/A Primers for genotyping, CRISPR/Cas9 genome editing, semi-quantitative PCR, RT-qPCR, RT-PCR, ISH, see Table S1 IDT N/A Software and algorithms Excel Microsoft N/A Fiji (ImageJ) Fiji https://fiji.sc FlowJo FlowJo https://www.flowjo.com/ Prism7 GraphPad Software GraphPad Software Imaris X Oxford Instruments N/A CellQuest software BD Bioscience N/A CytExpert software Beckman Coulter N/A Chopchop N/A https://chopchop.cbu.uib.no/ SnapGene GSL Biotech LLC N/A Ensembl EMBL-EBI https://www.ensembl.org/ index.html Other LSM700 Confocal Microscope Zeiss N/A Leica fluorescent stereomicroscope MZ 16 FA Leica N/A Leica SP8 confocal microscope Leica N/A Aria III flow cytometer BD Bioscience N/A FACSCalibur BD Bioscience N/A Jazz BD FACSJazz BD Bioscience N/A FACSDiva BD Bioscience N/A BD Influx Cell Sorter BD Bioscience N/A CytoFLEX benchtop flow cytometer Beckman Coulter N/A Hamamatsu Flash4 LT digital camera Hamamatsu N/A BIO-RAD C1000 Touch Thermal Cycler, CFX384TM Real-Time System BIO-RAD N/A ThermalSealRTTM Greiner Cat#: 676044 SAPPHIRE MICROPLATE, 384 WELL Greiner Cat#: 785201 Mmessage Mmachine Sp6 Kit Thermo Fisher Scientific Cat#: 10086184 Four-well imaging dish (Muwells) (Weijts et al., 2017) N/A Cell strainers 40 um Blue VWR Cat#: 734-0002 Adhesion slides, SuperFrost Plus VWR Cat#: 631-0108 Durapore Membrane Filters (0.65 mm) Millipore Cat#: DVPP02500 ..

    Flow Cytometry:

    Article Title: CLASP2 safeguards hematopoietic stem cell properties during mouse and fish development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES ThermoFisher Cat#: 15630080 Protease Inhibitor Cocktail Thermo Scientific Cat#: A32955 Bafilomycin A1 (BafA, 100nM) Invitrogen Cat#: tlrl-baf1 Tunicamycin Sigma-Aldrich Cat#: T7765 Fetal calf serum Sigma Cat#: F7524 L-Glutamine 200 mM (100ml) Life Technologies Cat#: 25030-024 L-Glutamine Invitrogen Cat#: 25030-032 Sodium pyruvate ThermoFisher Cat#: 11360070 Sodium pyruvate Invitrogen Cat#: 11360-039 2 -mercaptoethanol (0.1mM) Gibco Cat#: 11528926 Non-fat powder milk Sigma Cat#: 70166-500G PVDF membranes Millipore Cat#: IPVH00010 LIF Sigma-Aldrich Cat#: PD0325901 CHIR Tocris Biochemicals Cat#: 4423-10 PD Stemgent Cat#: 04-0006 Endo H NEB Cat#: P0702S Pngase F NEB Cat#: P0704S IRDyes 800CW and 600CW Li-cor N/A Enhanced chemiluminescence detection (ECL) GE Healthcare Cat#: RPN2236 Fluorescent detection (Odyssey Imaging System Licor Cat#: 926-68071 (goat anti-rabbit, IR dye 680 CW) Cat#: 926-32210 (goat anti-mouse, IR dye 800 CW) Ambion MEGAscript T7 transcription kit Life Technologies Cat#: AM1333 pCS2-nCas9 n plasmid Addgene Cat#: 47929 Clasp2 morpholino GeneTools, LCC N/A pCS2+ plasmid Kind gift from Prof. den Hertog (Hubrecht Institute) N/A BD Cytofix/CytopermTM BD Bioscience Cat#: 554714 RNeasy Mini kit Qiagen Cat#: 74104 SuperScript III Reverse Transcriptase Life Technologies Cat#: 18080044 SuperScript IV Reverse Transcriptase Thermo Fisher Cat#: 18-090-050 GFP-GM130 mRNA (Pouthas et al., 2008) mRNA kindly provided by Prof. Bakkers (Hubrecht Institute) N/A Experimental models: Organisms/strains Mouse: C57BL/6J (CD45.2) The Jackson Laboratory JAX: 000664 Mouse: Clasp2+/- (C57BL/6) Kind gift from Prof. Galjart (ErasmusMC, Rotterdam, NL) (Drabek et al., 2012) Zebrafish: mpeg1.4:mCherry/mpx:eGFP This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-4bp] This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-10bp] This paper N/A Zebrafish: clasp2mut[-4bp] This paper N/A Zebrafish: clasp2mut[-10bp] This paper N/A Zebrafish: AB EZRC Item #: 1175.1 Zebrafish: TL EZRC Item #: 1174.1 (Continued on next page) e3 Cell Reports 39, 110957, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MS-5 stromal line Kind gift from Drs. Dusanter and Lauret (Cochin Institute) N/A Oligonucleotides Morpholinos GeneTools, LCC N/A Primers for genotyping, CRISPR/Cas9 genome editing, semi-quantitative PCR, RT-qPCR, RT-PCR, ISH, see Table S1 IDT N/A Software and algorithms Excel Microsoft N/A Fiji (ImageJ) Fiji https://fiji.sc FlowJo FlowJo https://www.flowjo.com/ Prism7 GraphPad Software GraphPad Software Imaris X Oxford Instruments N/A CellQuest software BD Bioscience N/A CytExpert software Beckman Coulter N/A Chopchop N/A https://chopchop.cbu.uib.no/ SnapGene GSL Biotech LLC N/A Ensembl EMBL-EBI https://www.ensembl.org/ index.html Other LSM700 Confocal Microscope Zeiss N/A Leica fluorescent stereomicroscope MZ 16 FA Leica N/A Leica SP8 confocal microscope Leica N/A Aria III flow cytometer BD Bioscience N/A FACSCalibur BD Bioscience N/A Jazz BD FACSJazz BD Bioscience N/A FACSDiva BD Bioscience N/A BD Influx Cell Sorter BD Bioscience N/A CytoFLEX benchtop flow cytometer Beckman Coulter N/A Hamamatsu Flash4 LT digital camera Hamamatsu N/A BIO-RAD C1000 Touch Thermal Cycler, CFX384TM Real-Time System BIO-RAD N/A ThermalSealRTTM Greiner Cat#: 676044 SAPPHIRE MICROPLATE, 384 WELL Greiner Cat#: 785201 Mmessage Mmachine Sp6 Kit Thermo Fisher Scientific Cat#: 10086184 Four-well imaging dish (Muwells) (Weijts et al., 2017) N/A Cell strainers 40 um Blue VWR Cat#: 734-0002 Adhesion slides, SuperFrost Plus VWR Cat#: 631-0108 Durapore Membrane Filters (0.65 mm) Millipore Cat#: DVPP02500 ..

    Imaging:

    Article Title: CLASP2 safeguards hematopoietic stem cell properties during mouse and fish development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES ThermoFisher Cat#: 15630080 Protease Inhibitor Cocktail Thermo Scientific Cat#: A32955 Bafilomycin A1 (BafA, 100nM) Invitrogen Cat#: tlrl-baf1 Tunicamycin Sigma-Aldrich Cat#: T7765 Fetal calf serum Sigma Cat#: F7524 L-Glutamine 200 mM (100ml) Life Technologies Cat#: 25030-024 L-Glutamine Invitrogen Cat#: 25030-032 Sodium pyruvate ThermoFisher Cat#: 11360070 Sodium pyruvate Invitrogen Cat#: 11360-039 2 -mercaptoethanol (0.1mM) Gibco Cat#: 11528926 Non-fat powder milk Sigma Cat#: 70166-500G PVDF membranes Millipore Cat#: IPVH00010 LIF Sigma-Aldrich Cat#: PD0325901 CHIR Tocris Biochemicals Cat#: 4423-10 PD Stemgent Cat#: 04-0006 Endo H NEB Cat#: P0702S Pngase F NEB Cat#: P0704S IRDyes 800CW and 600CW Li-cor N/A Enhanced chemiluminescence detection (ECL) GE Healthcare Cat#: RPN2236 Fluorescent detection (Odyssey Imaging System Licor Cat#: 926-68071 (goat anti-rabbit, IR dye 680 CW) Cat#: 926-32210 (goat anti-mouse, IR dye 800 CW) Ambion MEGAscript T7 transcription kit Life Technologies Cat#: AM1333 pCS2-nCas9 n plasmid Addgene Cat#: 47929 Clasp2 morpholino GeneTools, LCC N/A pCS2+ plasmid Kind gift from Prof. den Hertog (Hubrecht Institute) N/A BD Cytofix/CytopermTM BD Bioscience Cat#: 554714 RNeasy Mini kit Qiagen Cat#: 74104 SuperScript III Reverse Transcriptase Life Technologies Cat#: 18080044 SuperScript IV Reverse Transcriptase Thermo Fisher Cat#: 18-090-050 GFP-GM130 mRNA (Pouthas et al., 2008) mRNA kindly provided by Prof. Bakkers (Hubrecht Institute) N/A Experimental models: Organisms/strains Mouse: C57BL/6J (CD45.2) The Jackson Laboratory JAX: 000664 Mouse: Clasp2+/- (C57BL/6) Kind gift from Prof. Galjart (ErasmusMC, Rotterdam, NL) (Drabek et al., 2012) Zebrafish: mpeg1.4:mCherry/mpx:eGFP This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-4bp] This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-10bp] This paper N/A Zebrafish: clasp2mut[-4bp] This paper N/A Zebrafish: clasp2mut[-10bp] This paper N/A Zebrafish: AB EZRC Item #: 1175.1 Zebrafish: TL EZRC Item #: 1174.1 (Continued on next page) e3 Cell Reports 39, 110957, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MS-5 stromal line Kind gift from Drs. Dusanter and Lauret (Cochin Institute) N/A Oligonucleotides Morpholinos GeneTools, LCC N/A Primers for genotyping, CRISPR/Cas9 genome editing, semi-quantitative PCR, RT-qPCR, RT-PCR, ISH, see Table S1 IDT N/A Software and algorithms Excel Microsoft N/A Fiji (ImageJ) Fiji https://fiji.sc FlowJo FlowJo https://www.flowjo.com/ Prism7 GraphPad Software GraphPad Software Imaris X Oxford Instruments N/A CellQuest software BD Bioscience N/A CytExpert software Beckman Coulter N/A Chopchop N/A https://chopchop.cbu.uib.no/ SnapGene GSL Biotech LLC N/A Ensembl EMBL-EBI https://www.ensembl.org/ index.html Other LSM700 Confocal Microscope Zeiss N/A Leica fluorescent stereomicroscope MZ 16 FA Leica N/A Leica SP8 confocal microscope Leica N/A Aria III flow cytometer BD Bioscience N/A FACSCalibur BD Bioscience N/A Jazz BD FACSJazz BD Bioscience N/A FACSDiva BD Bioscience N/A BD Influx Cell Sorter BD Bioscience N/A CytoFLEX benchtop flow cytometer Beckman Coulter N/A Hamamatsu Flash4 LT digital camera Hamamatsu N/A BIO-RAD C1000 Touch Thermal Cycler, CFX384TM Real-Time System BIO-RAD N/A ThermalSealRTTM Greiner Cat#: 676044 SAPPHIRE MICROPLATE, 384 WELL Greiner Cat#: 785201 Mmessage Mmachine Sp6 Kit Thermo Fisher Scientific Cat#: 10086184 Four-well imaging dish (Muwells) (Weijts et al., 2017) N/A Cell strainers 40 um Blue VWR Cat#: 734-0002 Adhesion slides, SuperFrost Plus VWR Cat#: 631-0108 Durapore Membrane Filters (0.65 mm) Millipore Cat#: DVPP02500 ..

    Membrane:

    Article Title: CLASP2 safeguards hematopoietic stem cell properties during mouse and fish development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES ThermoFisher Cat#: 15630080 Protease Inhibitor Cocktail Thermo Scientific Cat#: A32955 Bafilomycin A1 (BafA, 100nM) Invitrogen Cat#: tlrl-baf1 Tunicamycin Sigma-Aldrich Cat#: T7765 Fetal calf serum Sigma Cat#: F7524 L-Glutamine 200 mM (100ml) Life Technologies Cat#: 25030-024 L-Glutamine Invitrogen Cat#: 25030-032 Sodium pyruvate ThermoFisher Cat#: 11360070 Sodium pyruvate Invitrogen Cat#: 11360-039 2 -mercaptoethanol (0.1mM) Gibco Cat#: 11528926 Non-fat powder milk Sigma Cat#: 70166-500G PVDF membranes Millipore Cat#: IPVH00010 LIF Sigma-Aldrich Cat#: PD0325901 CHIR Tocris Biochemicals Cat#: 4423-10 PD Stemgent Cat#: 04-0006 Endo H NEB Cat#: P0702S Pngase F NEB Cat#: P0704S IRDyes 800CW and 600CW Li-cor N/A Enhanced chemiluminescence detection (ECL) GE Healthcare Cat#: RPN2236 Fluorescent detection (Odyssey Imaging System Licor Cat#: 926-68071 (goat anti-rabbit, IR dye 680 CW) Cat#: 926-32210 (goat anti-mouse, IR dye 800 CW) Ambion MEGAscript T7 transcription kit Life Technologies Cat#: AM1333 pCS2-nCas9 n plasmid Addgene Cat#: 47929 Clasp2 morpholino GeneTools, LCC N/A pCS2+ plasmid Kind gift from Prof. den Hertog (Hubrecht Institute) N/A BD Cytofix/CytopermTM BD Bioscience Cat#: 554714 RNeasy Mini kit Qiagen Cat#: 74104 SuperScript III Reverse Transcriptase Life Technologies Cat#: 18080044 SuperScript IV Reverse Transcriptase Thermo Fisher Cat#: 18-090-050 GFP-GM130 mRNA (Pouthas et al., 2008) mRNA kindly provided by Prof. Bakkers (Hubrecht Institute) N/A Experimental models: Organisms/strains Mouse: C57BL/6J (CD45.2) The Jackson Laboratory JAX: 000664 Mouse: Clasp2+/- (C57BL/6) Kind gift from Prof. Galjart (ErasmusMC, Rotterdam, NL) (Drabek et al., 2012) Zebrafish: mpeg1.4:mCherry/mpx:eGFP This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-4bp] This paper N/A Zebrafish: cd41:eGFP/kdrl:mcherry/ clasp2mut[-10bp] This paper N/A Zebrafish: clasp2mut[-4bp] This paper N/A Zebrafish: clasp2mut[-10bp] This paper N/A Zebrafish: AB EZRC Item #: 1175.1 Zebrafish: TL EZRC Item #: 1174.1 (Continued on next page) e3 Cell Reports 39, 110957, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MS-5 stromal line Kind gift from Drs. Dusanter and Lauret (Cochin Institute) N/A Oligonucleotides Morpholinos GeneTools, LCC N/A Primers for genotyping, CRISPR/Cas9 genome editing, semi-quantitative PCR, RT-qPCR, RT-PCR, ISH, see Table S1 IDT N/A Software and algorithms Excel Microsoft N/A Fiji (ImageJ) Fiji https://fiji.sc FlowJo FlowJo https://www.flowjo.com/ Prism7 GraphPad Software GraphPad Software Imaris X Oxford Instruments N/A CellQuest software BD Bioscience N/A CytExpert software Beckman Coulter N/A Chopchop N/A https://chopchop.cbu.uib.no/ SnapGene GSL Biotech LLC N/A Ensembl EMBL-EBI https://www.ensembl.org/ index.html Other LSM700 Confocal Microscope Zeiss N/A Leica fluorescent stereomicroscope MZ 16 FA Leica N/A Leica SP8 confocal microscope Leica N/A Aria III flow cytometer BD Bioscience N/A FACSCalibur BD Bioscience N/A Jazz BD FACSJazz BD Bioscience N/A FACSDiva BD Bioscience N/A BD Influx Cell Sorter BD Bioscience N/A CytoFLEX benchtop flow cytometer Beckman Coulter N/A Hamamatsu Flash4 LT digital camera Hamamatsu N/A BIO-RAD C1000 Touch Thermal Cycler, CFX384TM Real-Time System BIO-RAD N/A ThermalSealRTTM Greiner Cat#: 676044 SAPPHIRE MICROPLATE, 384 WELL Greiner Cat#: 785201 Mmessage Mmachine Sp6 Kit Thermo Fisher Scientific Cat#: 10086184 Four-well imaging dish (Muwells) (Weijts et al., 2017) N/A Cell strainers 40 um Blue VWR Cat#: 734-0002 Adhesion slides, SuperFrost Plus VWR Cat#: 631-0108 Durapore Membrane Filters (0.65 mm) Millipore Cat#: DVPP02500 ..

    Real-time Polymerase Chain Reaction:

    Article Title: Downregulation of TASK-3 Channel Induces Senescence in Granulosa Cells of Bovine Cystic Ovarian Follicles
    Article Snippet: Resultant bands were extracted and underwent direct sequencing using an ABI PRISM ® 3100-Avant Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). .. For real-time PCR, a combination of the Topreal TM qPCR 2X PreMIX kit (Enzynomics, Daejeon, Republic of Korea), sapphire microplate, 96 wells, (Greiner bio-one, Kremsmünster, Austria), and the Light Cycler ® 480 II System (Roche, Rotkreuz, Switzerland) was utilized. ..



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